FIG 1.

pqqF is not required for host immunity suppression in P. simiae WCS417. (A) While loss of function of P. aeruginosa PAO1 pqqF results in inability to suppress expression of CYP71A12_pro:GUS expression, the P. simiae WCS417 pqqF-deficient mutant retains the ability to suppress flg22-induced CYP71A12_pro:GUS expression. (B) Deletion or disruption of pqqF results in a pH increase in WCS417 and P. aeruginosa PAO1. Individual data are the result of a single well of seedling exudates. Statistics were determined by using one-way ANOVA and Tukey’s HSD. Error bars represent mean ± SD, and letters indicate differences at P < 0.05. (C) Percent of GUS-stained CYP71A12_pro:GUS roots in the presence of flg22 under a pH gradient (black dots). pH of WCS417 (green squares) and the pqqF mutant fully suppress flg22-triggered CYP71A12_pro:GUS expression. Each dot represents the percent of blue roots from 1 to 3 wells of a temporal experiment containing 3 to 4 roots per well. All experiments were independently repeated at least 3 times.