FIG 3.

DNA binding of Clr-cAMP and Clr-cGMP at the SMc05008 promoter. (A) The promoter region of SMc05008 has three possible DNA binding sites (colored), with one mismatch each. Mutations indicated in red were introduced at four different positions (M1 to M4). Nucleotides not matching the consensus Clr binding sequence are shown in bold. (B) A heat map shows the cAMP- or cGMP-induced fold change of EGFP-mediated fluorescence compared to that of noninducing conditions (no cNMP added). Asterisks indicate significant changes in the EGFP-mediated fluorescence (Table S2).