FIG 4.

Functional characterization of the pol30-K127R mutation. (A) Genetic interaction between pol30-K127R and pol30-A171D. Experimental conditions are as described in Fig. 2. (B) Western blot analysis of cellular PCNA levels. Pol30 variants are as indicated on the top panel. Pgk1 was used as a loading control. (C) Physical interaction between Srs2 and Pol30 amino acid substitutions by a Y2H assay. Experimental conditions are as described in Fig. 3B, and the plates were incubated at 30°C for 3 days before photography.