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Phenotypes of selected pol30 amino acid substitution mutants. (A) Physical interaction between pol30 mutations and Srs2 or Rad30 by a Y2H assay. Experimental conditions were as described in Fig. 3. (B,C) Phenotypes of pol30 mutants in rescuing rad5Δ (B) and rad18Δ (C) mutants from killing by DNA-damaging agents in a serial dilution assay. Experimental conditions were as described in Fig. 2. All plates were incubated at 30°C for 2 days before photography.
