Skip to main content
. Author manuscript; available in PMC: 2023 Apr 25.
Published in final edited form as: Nat Chem Biol. 2022 Sep 29;19(3):275–283. doi: 10.1038/s41589-022-01149-6

Extended Data Fig. 9 |. Targeted S-nitrosylation of ACE2 by NMT5 in the presence of envelope (E) viroporin protein.

Extended Data Fig. 9 |

Kinetic analysis of S-nitrosylation of ACE2. E protein plasmid was transiently transfected into HEK293 cells stably-expressing Spike protein cells. Subsequently, the cells were harvested and plated onto ACE2-expressing HeLa cells in the presence or absence of 20 μM NMT5. At the indicated timepoints, cells were subjected to biotin-switch assay. Ordinate shows ratio of SNO-ACE2/total input ACE2 protein above the baseline value, defined as 1.0. Data are mean + s.e.m. by two-way ANOVA with Fisher’s LSD test. n = 5 biological replicates.