TABLE 1.
SNV calling sensitivitya
| Sample | Dilution factor | IT in pfu/mL | Coverage | SNV |
|---|---|---|---|---|
| MT RNA (n:428) | 1 | NA | 100.00% | 426 |
| 4 | NA | 100.00% | 427 | |
| 16 | NA | 100.00% | 427 | |
| 64 | NA | 100.00% | 426 | |
| 256 | NA | 100.00% | 427 | |
| 1,024 | NA | 100.00% | 428 | |
| 4,096 | NA | 99.84% | 423 | |
| 16,384 | NA | 98.27% | 423 | |
| WT virus (n:8) | 1 | 115,000 | 100.00% | 8 |
| 4 | 28,750 | 100.00% | 8 | |
| 16 | 7,188 | 100.00% | 8 | |
| 64 | 1,797 | 100.00% | 8 | |
| 256 | 449 | 100.00% | 8 | |
| 1,024 | 112 | 100.00% | 8 | |
| 4,096 | 28 | 100.00% | 8 | |
| 16,384 | 7 | 100.00% | 8 |
a
SNV refers to the number of correctly called SNVs relative to SARS-CoV2/hu/CHN/Wuhan-Hu-1/2019 (NC_045512) for either an artificial RNA with 428-point mutations or WT virus strain 2019-nCoV/USA-WA1/2020(BEI resources, Cat. No. NR-52281). The dilution factor shows the dilution. It refers to infectious titer in plaque-forming units/mL—coverage refers to coverage at 1×. The raw reads are available as bio project.