FIG 1.

Intradermal BCG elicits greater mycobacteria-specific CD4 T cell responses in the airways compared to intragastric gavage BCG. (A) Four animals were intradermally vaccinated with BCG, 2 at a higher dose and 2 at a lower dose, and 4 animals were vaccinated by intragastric gavage, 2 at a higher dose and 2 at a lower dose. Animals were necropsied at 11 or 12 weeks postvaccination. (B) Example FACS plots of TNF and IFNγ staining on CD4 T cells from the BAL following in vitro stimulation with PPD or MTB300 MHC-II peptide megapool. (C) Graph showing percentage of BAL CD4 T cells that are TNF⁺ following in vitro PPD stimulation in each animal, and summary graph showing each animal’s area under the curve (AUC). (D) Graph showing percentage of BAL CD4 T cells that are TNF⁺ following in vitro MTB300 stimulation in each animal, and summary graph showing each animal’s area under the curve (AUC). (E) Graph showing percentage of CD4 T cells from PBMCs that are TNF⁺ following in vitro PPD stimulation in each animal, and summary graph showing each animal’s area under the curve (AUC). (F) Graph showing percentage of CD4 T cells from PBMCs that are TNF⁺ following in vitro MTB300 stimulation in each animal, and summary graph showing each animal’s area under the curve (AUC). (G) Ratio of PPD to MTB300-specific CD4 T cells from each animal in BAL and blood. **, P < 0.01.