FIG 1.

MXRA8 is required for optimal RRV disease development and viral replication. (A and B) WT and MXRA8^−/− mice (n = 7 per group) were infected with RRV at 10⁴ PFU or mock infected with PBS. Mouse disease and weight were monitored daily up to 10 dpi. The data are representative of two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (using two-way ANOVA with Bonferroni posttest). (C) Mouse sera of infected WT and MXRA8^−/− mice were collected at 1, 3, and 5 dpi and processed for plaque assay. The data, representative of two independent experiments, are presented as means ± the standard errors of the mean (SEM). ****, P < 0.0001 (using two-way ANOVA with the Bonferroni posttest). (D to I) Quadriceps and ankles of the infected WT and MXRA8^−/− mice were harvested at 3, 7, and 10 dpi. Viral titers in these tissues were determined by plaque assay. Dots represent individual animals (n = 7). The data, representative of two independent experiments, are presented as means ± the SEM. ***, P < 0.001 (using a t test with Mann-Whitney posttest).