Pangenome of Rothia. This pangenome was generated from 9 reference genomes and 14 draft genome sequences of the strains used in this study using the anvi’o 7 pangenome pipeline. Each arc represents a single genome and is colored based on its species-level identification, as indicated by the key below. Genomes are ordered based on average nucleotide identity (ANI; top right). In total, this pangenome contains 48,332 genes within 8,099 gene clusters. The presence or absence of a gene cluster within a given genome is indicated by opaque and transparent colors, respectively. Black bar indicates the set of single-copy core gene (SCG) clusters.