Impact of HGC1 deletion on virulence traits. Human endothelial cells were incubated with the indicated strains of C. albicans for 3 h, after which the extent of host cell damage was determined using a 51Cr release assay. Results are the mean from three independent experiments, each performed in triplicate. Error bars denote standard deviation. Data were analyzed using Bonferroni’s multicomparison test (ns, P > 0.05; *, P < 0.05; ***, P < 0.001).