FIG 5.

Infection of IFNγ-stimulated HFFs with candidate gene knockout parasites leads to increased host cell death due to early parasite egress. HFFs were prestimulated with IFNγ (10 to 20 U/mL) and subsequently infected with indicated parasite strains (in RH background) for 24 h, after which (A) LDH release in the supernatant was measured. Plotted is the % LDH release compared to maximal LDH release (after triton treatment of cells). (B) The number of parasites/vacuole were counted. (C) As in panel A, but 6 h after infection, 1 μM the PKG inhibitor Compound 1 was added. Indicated are means ± SEM from 3 (A, B, and C for Δgra70+GRA70, and Δgra66) or 6 biological replicates (C for WT and Δgra70). Two-way ANOVA followed by Tukey’s multiple-comparison test was used for statistical analysis. *, P < 0.05; **, P < 0.01.