TABLE 2.
Immunoprecipitation of GRA70 and GRA57a
| ToxoDB_IDb | Localization | Description | GRA70 | GRA57 | 263560 | GRA35 | GRA15 |
|---|---|---|---|---|---|---|---|
| TGME49_217680 | Dense granules | GRA57 | 43 | 169 | 1 | 1 | 9 |
| TGME49_309600 | Dense granules | Hypothetical protein /GRA71 | 15 | 70 | 0 | 0 | 8 |
| TGME49_249990 | Dense granules | Putative microtubule-binding protein/GRA70 | 71 | 56 | 0 | 0 | 7 |
| TGME49_212300 | Dense granules | GRA32 | 79 | 22 | 0 | 0 | 4 |
| TGME49_279100 | Dense granules | MAF1/GRA67 | 0 | 11 | 0 | 0 | 0 |
| TGME49_320490 | Dense granules | N-acylphosphatidylethanolamine-hydrolyzing phospholipase D/ GRA66 | 0 | 9 | 0 | 0 | 1 |
| TGME49_311720 | Er 2 | Chaperonin protein BiP | 9 | 72 | 3 | 2 | 14 |
| TGME49_249900 | Mitochondrion − membranes | Putative adenine nucleotide translocator | 0 | 12 | 0 | 2 | 2 |
| TGME49_204400 | Mitochondrion − membranes | TgATPα | 0 | 9 | 0 | 0 | 0 |
| TGME49_261950 | Mitochondrion − membranes | TgATPβ | 0 | 7 | 0 | 0 | 0 |
| TGME49_278870 | PM − peripheral 2 | TgMyoF | 3 | 9 | 2 | 1 | 0 |
| TGME49_252430 | PM − peripheral 2 | Putative START-2 domain protein | 0 | 7 | 0 | 1 | 1 |
| TGME49_301440 | Peripheral/cytosol/nucleus | CDPK1 | 0 | 13 | 0 | 0 | 0 |
| TGVEG_442220 | Rhoptries 1 | ROP5 | 0 | 11 | 0 | 0 | 0 |
a
Listed are the number of unique peptides that were detected by mass-spectrometry after immunoprecipitation of 3xHA-tagged GRA70 or GRA57 with anti-HA magnetic beads.
b
Toxoplasma proteins predicted to be secretory proteins or associated with membranes and that had at least 7 unique peptides and at least a 4-fold enrichment compared to immunoprecipitations of the control proteins (GRA35, GRA15, and the GRA TGGT1_263560). Proteins listed in bold were identified as genes that determine fitness in IFNγ-stimulated HFFs (Table 1). The entire data set is presented in Table S5.