Figure 2. DNMT3A knockdown reduced the proliferation, migration, and invasion of pancreatic cancer cells, as well as tumor formation in nude mice.

Plasmid sh-DNMT3A was transfected into PANC-1 and Capan-1 cells for silencing DNMT3A expression. Cells were divided into two groups: sh-NC and sh-DNMT3A. (A, B) The knockdown efficiency was measured by qRT-PCR and Western blot. (C) EdU assay was performed to detect the proliferation of treated cells. (D, E) The migration and invasion capacities of treated cells were evaluated by wound healing and transwell assays. PANC-1 and Capan-1 cells stably expressed sh-NC or sh-DNMT3A and were subcutaneously injected into BALB/c nude mice to establish xenograft mice model. (F) The measurement of tumor volume and weight (n = 5). (G, H) DNMT3A expression in mice model after silencing DNMT3A. *P < 0.05, **P < 0.01, and ***P < 0.001.