Table 1.
Formulation and proximate analysis of the basal diet (g/kg diet).
| Ingredient | Content |
|---|---|
| Fish meal (70.2% crude protein) | 105.00 |
| Poultry by-product meal (66.5% crude protein) | 200.00 |
| Wheat meal (12.0% crude protein) | 150.00 |
| Gelatin (97.5% crude protein) | 40.00 |
| Yeast powder (58.2% crude protein) | 30.00 |
| Wheat bran (18.6% crude protein) | 110.00 |
| Fish oil | 25.00 |
| Soybean oil | 30.00 |
| Vitamin mixture a | 15.00 |
| Mineral mixture b | 15.00 |
| Amino acid mixture c | 150.00 |
| Calcium dihydrogen phosphate | 10.00 |
| Choline chloride | 10.00 |
| Cellulose | 99.00 |
| Dimethyl-β-propiothetin | 1.00 |
| Composition (% dry weight basis) | |
| Moisture | 7.00 |
| Crude protein | 48.56 |
| Crude lipid | 10.38 |
| Crude ash | 10.04 |
| Carbohydrate1 | 24.02 |
| Gross energy2, kJ/g | 19.72 |
a.Vitamin premix (mg/kg diet): vitamin B1, 25 mg; vitamin B2, 45 mg; vitamin B12, 10 mg; vitamin B6, 20 mg; vitamin K, 10 mg; vitamin A, 32 mg; vitamin C, 2000 mg; vitamin D3, 5 mg; vitamin E, 240 mg; niacin acid, 200 mg; folic acid, 20 mg; biotin, 60 mg; calcium pantothenate, 60 mg; micocrystalline cellulose 12273 mg.
b Mineral premix (mg/kg diet): inositol, 800 mg; MgSO4·H2O, 1200 mg; CuSO4·5H2O, 10 mg; FeSO4·H2O, 80 mg; ZnSO4·H2O, 50 mg; MnSO4·H2O, 45 mg; CoCl2·6H2O, 50 mg; Ca(IO3)2, 60 mg; Na2SeO3, 20 mg; zeolite powder, 12685 mg.
c Amino acid mixture (g/kg diet): arginine, 9.98 g; histidine, 3.37 g; isoleucine, 9.91 g; leucine, 18.60 g; lysine, 23.52 g; methionine, 6.27 g; cysteine, 10.27 g; phenylalanine, 7.57 g; threonine, 10.79 g; valine, 8.48 g; aspartic acid, 28 g; glycine, 14 g.
1 Carbohydrate (%) = 100 - (% crude protein + % crude lipid + % moisture + % ash).
2 Calculated based on 17.2 kJ g−1 carbohydrate; 23.6 kJ g−1 protein and 39.5 kJ g−1 lipid according to the method described in a previous study (43).