Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. rerio) | Tg(flt4:mCitrine)hu7135 | van Impel et al., 2014 | ZFIN: hu7135 | |
| Genetic reagent (D. rerio) | Tg(flt1enh:tdTomato)hu5333 | Bussmann and Schulte-Merker, 2011 | ZFIN: hu5333 | |
| Genetic reagent (D. rerio) | Tg(lyve1:DsRed2)nz101 | Okuda et al., 2012 | ZFIN: nz101 | |
| Genetic reagent (D. rerio) | Tg(UAS:RFP)nkuasrfp1a | Asakawa et al., 2008 | ZFIN: nkuasrfp1a | |
| Genetic reagent (D. rerio) | Tg(vegfc:Gal4FF)mu402 | Wang et al., 2020 | ZFIN: mu402 | |
| Genetic reagent (D. rerio) | Tg(svep1:GAL4FF)hu8885 | Karpanen et al., 2017 | ZFIN: hu8885 | |
| Genetic reagent (D. rerio) | adamts3hu10891 | Wang et al., 2020 | ZFIN: hu10891 | |
| Genetic reagent (D. rerio) | adamts14hu11304 | Wang et al., 2020 | ZFIN: hu11304 | |
| Genetic reagent (D. rerio) | vegfchu6410 | Helker et al., 2013; Le Guen et al., 2014 | ZFIN: hu6410 | |
| Genetic reagent (D. rerio) | ccbe1hu10965 | Kok et al., 2015 | ZFIN: hu10965 | |
| Genetic reagent (D. rerio) | svep1hu6123 | Karpanen et al., 2017 | ZFIN: hu6123 | |
| Genetic reagent (D. rerio) | svep1hu4767 | Karpanen et al., 2017 | ZFIN: hu4767 | |
| Genetic reagent (D. rerio) | tie1bns208 | Carlantoni et al., 2021 | ZFIN: bns208 | |
| Genetic reagent (D. rerio) | tie2hu1667 | Gjini et al., 2011 | ZFIN: hu1667 | |
| Genetic reagent (D. rerio) | TgBAC(apln:EGFP)bns157 | Helker et al., 2020 | ZFIN: bns157 | |
| Cell line (Homo sapiens) | 293T HEK cells | Roukens et al., 2015 | ||
| Cell line (Homo sapiens) | HEK293 EBNA | Manuel Koch | ||
| Transfected construct (D. rerio) | zfTie1-HA in PCS2+ | This paper | Provided by Naoki Mochizuki | |
| Transfected construct (D. rerio) | zfTie2-HA in PCS2+ | This paper | zfTie2 cDNA provided by Naoki Mochizuki | |
| Transfected construct (D. rerio) | zfSvep1-HIS in PCS2+ | This paper | ||
| Transfected construct (Homo sapiens) | TIE1-HA in PCEP4 | This paper | TIE1 cDNA provided by Hellmut Augustin | |
| Transfected construct (Homo sapiens) | TIE2-HA in PCEP4 | This paper | Provided by Manuel Koch | |
| Transfected construct (Homo sapiens) | SVEP1-Strep II in PCEP4 | This paper | Provided by Manuel Koch | |
| Transfected construct (Mus musculus) | mouse nope ectodomain with Fc tag strep | This paper | Provided by Manuel Koch | |
| Antibody | anti-HA (rat monoclonal) | Roche | 11867423001 | 1:10,000 |
| Antibody | anti-rat (donkey polyclonal) | Invitrogen | # A18745 | 1:15,000 |
| Antibody | anti-HIS (rabbit polyclonal) | Invitrogen | # PA1-983B | 1:250 |
| Antibody | anti-HIS (mouse monoclonal) | Invitrogen | # MA1-135 | 1:250 |
| Antibody | anti-mouse (goat polyclonal) | dako | P0447 | 1:4000 |
| Antibody | anti-DIG primary antibody (sheep polyclonal) | Roche | 11093274910 | 1:2000 |
| Recombinant DNA reagent | apelin in pGEM-t-easy | Provided by Christian Helker | For in situ probe generation | |
| Peptide, recombinant protein | SVEP1-Strep II Purified recombinant protein |
This paper | Provided by Manuel Koch | |
| Chemical compound, drug | DIG RNA Labeling Mix | Roche | 11277073910 | |
| Chemical compound, drug | Lipofectamin 2000 transfection reagent | Thermo Fisher Scientific | 11668030 | |
| Chemical compound, drug | FuGENE HD Transfection Reagen | Promega | E2311 | |
| Chemical compound, drug | T4 Ligase | Thermo Fisher Scientific | EL0012 | |
| Chemical compound, drug | FCS | Merck Chemicals GmbH | F7524 | |
| Chemical compound, drug | Q5 Hot Start High-Fidelity DNA Polymerase | New England Biolabs GmbH | M0493 | |
| Chemical compound, drug | DMEM/F-12, GlutaMAX Supplement | Thermo Fisher Scientific | 10565018 | |
| Chemical compound, drug | Strep-Tactin Superflow high capacity resin | IBA Lifesciences GmbH | 2-1208-002 | |
| Software, algorithm | GraphPad Prism 6 | GraphPad Software, USA | ||
| Software, algorithm | Fiji-ImageJ (version 1.52); Manual tracking plugin; StrackReg plugin |
DOI:10.1038/nmeth.2019; Fabrice Cordelières, Institut Curie, Orsay (France); DOI:10.1109/83.650848 |
RRID:SCR_002285 | |
| Software, algorithm | Python (version 3.8) | Python.org | RRID:SCR_008394 | Code available at https://github.com/MuensterImagingNetwork/Hussmann_et_al_2022 |
| Other | Strep-Tactin HRP conjugate | Iba-lifesciences | 2-1502-001 | 1:10,000 |