Table 1.

Major mutations detected from wastewater samples collected at a COVID-19 quarantine facility and municipal WWTPs.

		COVID-19 quarantine facility			WWTP A	WWTP B	WWTP A
		Oct. 27, 2020	Nov. 27, 2020	Dec. 24, 2020	Nov.19, 2020	Dec. 4, 2020	Jan. 7, 2021
Average depth		665,660	799,247	856,836	928,072	990,596	869,124
Mutation frequencya	N501Y	< 5 % (0.1 %)b	< 5 % (0.7)%	39.8 %	< 5 % (0.1 %)	5.9 %	12.4 %
	D614G	47.5 %	83.1 %	82.8 %	6.0 %	17.3 %	48.8 %
	H655Y	5.8 %	5.7 %	<5 % (2.8 %)a	7.6 %	5.4 %	12.1 %
	E484K	N.D.c	N.D.	N.D.	N.D.	N.D.	N.D.

^aThe mutation ratio at major mutation points of wastewater samples collected at a COVID-19 quarantine facility and municipal WWTPs (A and B). The percentages were calculated by dividing the number of reads that contain the specific mutation by the total reads of SARS-CoV-2.

^bWhen a mutation frequency was lower than 0.05 (5 %), the existence of the reads was confirmed with IGV. The abundance ratio is indicated in parentheses.

^cN.D., not detected.