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. 2023 Apr 25;13:6745. doi: 10.1038/s41598-023-33448-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cortical layer distribution and neuronal morphology of validation sequences VS33 and VS47 in coronal sections of the auditory cortex. (A, D) Example fluorescence microscopy image of a cortical slice depicting mTFP expression driven by VS33 (A) or VS47 (D). Six neocortical layers indicated on DAPI image. (B, E) Distribution of mTFP-expressing cells across cortical layers (mean across images ± SD) for VS33 (B) or VS47 (E). VS33 does not show layer-specificity, but VS47 drives expression mainly in layer5 of the cortex. (C, F) Example cell morphology tracings of neurons expressing EYFP after recombination byVS33-driven (C) or VS47-driven (F) Cre-recombinase. Both sequences drive expression in diverse morphological cell types.