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. 2023 Apr 25;3:38. doi: 10.1038/s43705-023-00245-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Flow chart of the experiment B Family level bacterial composition of the sample (left), including original fecal sample and its ex vivo cultures, grown anaerobically in 5 different media at incubation time of 6,12, and 24 h. Cultures are ordered according to their Jensen-Shannon divergence (D_JS) from the original fecal sample (upper axes, computed at the family level). ASV level bacterial composition of the sample (right), where each square represents one sample. Rainbow colored dots represent the relative abundance of individual ASVs. Samples are ordered by their Shannon index at the ASV level, computed in base 2 and shown above each square. C Comprehensive rank of 15 cultures, which are ordered according to the Euclidean (ASV level) and D_JS (family level) from the original fecal sample (rank from 1 to 15 in ascending order), and the Shannon index (rank from 1 to 15 in descending order), the smaller rank indicates higher similarity and diversity.