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Average dissociation constants (KD) for TrmB WT and variants binding to [3H]tRNAPhe in the presence and absence of 50 μM SAM
| TrmB variant | +SAM, μMa | −SAM, μM |
|---|---|---|
| TrmB WT | 2.1 ± 0.5 | 6.6 ± 1.9 |
| TrmB D144A | 0.8 ± 0.2 | 2.5 ± 0.7 |
| TrmB T217A | 2.6 ± 0.6 | 5.4 ± 1.4 |
| TrmB R26A | 5.1 ± 0.8 | 6.4 ± 2.5 |
| TrmB R155A | 5.8 ± 0.9 | 10 ± 2.8 |
TrmB binding to tRNA in the presence of SAM for 10 min results in m7G46 formation such that the affinity of TrmB to its product rather than its substrate is measured.
