Table 2.

Cycling conditions for Universal and Fast real-time PCR

Step	Universal condition (7500 and ViiA7)	Fast condition (7500 Fast, StepOnePlus, and QuantStudio 3)
1. First denaturing	95 °C, 10 min, 1 cycle	95 °C, 20 s, 1 cycle
2. Denature	95 °C, 15 s	95 °C, 3 s
3. Anneal	60 °C, 60 s	60 °C, 30 s
4. Repeat steps 2–3	Repeat 40 cycles	Repeat 40 cycles