Table 3.
Result of specificity test using real-time PCR method
| Classification | Common name (scientific name) | Ct value |
|---|---|---|
| Target species | Centipede (Scolopendra mutilans) | 18.83 ± 0.08a |
| Non-target species | Blister beetle (Hycleus cichorii) | ND |
| Earthworm (Lumbricina sp.) | ND | |
| Gecko (Gekko gecko) | ND | |
| Honeybee pupae (Apis mellifera) | ND | |
| Migratory locust (Lcusta migratoria) | ND | |
| Rhinoceros beetle larvae (Allomyrina dichotoma) | ND | |
| Rice grasshopper (Oxya japonica) | ND | |
| Silkworm (Bombyx mori) | ND | |
| Twin-star cricket (Gryllus bimaculatus) | ND |
ND not detected
aMean Ct value ± standard deviation obtained from triplicate reactions