Fig. 3.

Influence of sonication on post-consumer PET depolymerization in the presence of Humicola insolens cutinase (HiC). Experimental conditions: 3.2 vol% biocatalyst; 5 g L⁻¹ PET; phosphate buffer (pH = 8.0 and 0.1 mol L⁻¹); 200 rpm; 50 °C; 7 days. Sonication was applied for 10, 20, and 30 min in the enzyme's presence and 30 min before adding the biocatalyst