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. 2023 Apr 12;13:973871. doi: 10.3389/fonc.2023.973871

Figure 1.

Figure 1

High metastatic tumor EVs induce tumor cell adhesion to the lung and promote lung metastasis. (A) The experimental design of tumor EV and tumor cell injection. Tumor cells were injected after five injections of A375SM-EVs. After 24 h and 40 days, tumor cell signals were analyzed using an in vivo imaging system (IVIS). (B, C) Tumor cell luminescence intensity in the lungs of the control and A375SM-EV injected groups was detected using IVIS Spectrum 24 h after tumor injection (B). Quantitative analysis of luminescence intensity (Total flux) was shown in (C) (**P = 0.012, Wilcoxon test; n = 5 mice per group). (D) Tumor cell luminescence intensity in the control and A375SM-EV injected groups after 40 days from tumor injection was detected using IVIS Spectrum. Quantitative analysis of luminescence intensity (Total flux) was shown in (E) (**P = 0.037, Wilcoxon test; n = 5 mice per group). (F) Representative H-E staining images of lung metastases in the two groups. Arrows indicate tumor nodules. (G) The number of metastatic nodules in the whole lung tissue was quantified (**P = 0.026, Wilcoxon test; n = 5 mice per group).