Figure 2.

miR-1246 knockdown of tumor cells reduces lung metastasis. (A) Images of mice, 28 days after subcutaneous injection of Control-miR A375SM and Anti-miR-1246 A375SM. (B) miR-1246 levels in serum EVs from Control-miR A375SM or Anti-miR-1246 A375SM tumor-bearing mice. Sera were collected 28 days after subcutaneous injection of each tumor (**P = 0.037, Wilcoxon test; data are presented as mean ± SD; n = 3 real-time RT-PCR runs, n = 5 mice per group). (C) Tumor volume was assessed using the formula: (width² × length)/2 (mm³). Two-sided Student’s t-test. N.S.: not significant. P = 0.42 (D) Tumor cell fluorescence intensity (GFP signal) in the lungs was detected using IVIS Spectrum. Quantitative analysis of fluorescence intensity (Total flux) was shown in (E) (**P = 0.022, Wilcoxon test; n = 5 mice per group).