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. 2023 Apr 12;11:1108952. doi: 10.3389/fbioe.2023.1108952

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Copyright © 2023 Yao, Liao, Chen, Liu, Wu, Hao, Li, Wang, Fan, Yin, Liu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of pEVs. (A) The morphological characteristics of pEVs were detected by TEM. (B) The EVs markers (Alix, TSG101 and Syntenin-1) and non-EVs marker (ApoB) were detected by Western blotting. 20 uL sample or 20 μg protein from each fraction was loaded. (C) The pEVs were fluorescently labeled with PE-conjugated EVs mAbs (CD9, CD63 and CD81), and the positive ratio was detected by nFCM measurement.