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. 2023 Apr 12;11:1108952. doi: 10.3389/fbioe.2023.1108952

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In our study, three methods were used for isolating extracellular vesicles derived from pleural effusion (pEVs), including ultracentrifugation (UC), a combination of UC and size exclusion chromatography (UC-SEC) and a combination of UC and density gradient ultracentrifugation (UC-DGU), and followed with proteomics analysis. Our results show that the method of UC-SEC could significantly improve the purity of pEVs and improve the performance of proteomic analysis of pEVs. The exosomal proteins CD11C, HLA DPA1 and HLA DRB1 may act as potential markers of pEVs.