FIGURE 10.

Trehalose potentiates poly(I:C) mediated activation of HMC3. (A) HMC3 were treated with 0.15–15 mM of trehalose for 24 h and metabolic activity was assessed via XTT assay. (B) HMC3 were left untreated (UT) or treated with 0.15–15 mMof trehalose for 3 h and subsequently activated with 0.5 μg/mLof poly(I:C) for 24 h and (B) cell free supernatants were measured for IL-8 via ELISA. Data presented as mean ± SEM for n = 3. Statistical significance was calculated using two-way ANOVA with Tukey post hoc analysis: p ≤ 0.0001 (****). (C, E) Cell lysates were analyzed for MDA5 and RIGI expression via Western blot and (D, F) corresponding densitometry analysis was performed. Unrelated samples are annotated as UR. Western blot images are representative of n = 3. Densitometry data presented as mean ± SEM for n = 3 and statistical analysis was calculated using one-way ANOVA with Tukey post hoc analysis.