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. 2023 Apr 12;14:1102465. doi: 10.3389/fphar.2023.1102465

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Copyright © 2023 Wagner, Pehar, Yan and Kulka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AME-1 significantly increases expression of CD125, CXCR4, CD86, TLR4, and CD45. MFI histograms and corresponding bar graphs of HMC3 treated with 50–5,000 μg/mL of AME-1 for 24 h,labelled with (A, B) PE-CD125, (C, D) PE-CXCR4, (E, F) APCCD86, (G, H) FITC-TLR4 or (I, J) FITC-CD45 and analyzed via flow cytometry. Data shown in flow cytometry histograms is representative of three independent experiments (n = 3). Mean fluorescence intensity (MFI) values for all three experiments are presented as mean ± SEM forn = 3. Statistical significance was calculated using one-way ANOVA with Tukey post hoc analysis, p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.0001 (****).