Video 1.
TRM in proximity to soft palate taste buds. Ce3D imaging of VPEP-elicited OT-I T cells in the vicinity of isolated taste buds of the soft palate. Palatal tissue from a VPEP memory mouse was made optically transparent as previously described (Li et al., 2017). Cleared tissue was imaged by tiling Z-stacks with confocal laser scanning microscopy (Stelleris), and 3D reconstructions were produced using Imaris (Bitplane) software v9.2.1. Movement through the Z-stack is viewed by 60 µm extended sections at 10 frames per second. For orientation, the eighth palatine ridge is visible at 0:00:00, and isolated taste buds are visible between 0:00:00–0:00:02. Taste buds are identified by crescent morphology. Red, OT-I CD8+ T cells; blue, nonspecific labeling of the epithelium. Corresponding still images from this video are displayed in Fig. 4 E. Images are representative of two Ce3D soft palate specimens from VPEP memory mice.