Figure 4.
Dynamic changes of Itgadhi/Fcrl5hi macrophages in NASH progression
(A) Representative images for H&E, oil red O and PSR stained liver tissues of chow and HFD (week 8, 16 and 32) mice. The statistics of NAS and the area of oil red O and PSR were shown on the right. Scale bar, 100 μm.
(B) Immunofluorescence staining of Itgad+ macrophages in liver tissues of chow and HFD (week 8, 16, and 32) mice. Scale bar, 50 μm.
(C) The statistics of Itgad+ macrophages in liver tissues of chow and HFD (week 8, 16, and 32) mice.
(D) Relative mRNA levels of Itgad and Fcrl5 in liver tissues of chow and HFD (week 8, 16, and 32) mice.
(E) Percentage of Fcrl5+ cells in F4/80+CD11b+ double-positive macrophages isolated from liver tissues of chow and HFD (week 16 and 32) mice. The statistics were shown on the right. n = 3–5 per group. The data are expressed as mean ± SEM. Statistical significance is shown as ∗ for p < 0.05, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001, as evaluated by one-way ANOVA.