Fig. 4.

Female Nf1^+/− mice exhibit more anxious-like behavior. a In the open field test, mutant females spent less time in the center of the arena (***p < 0.001, comparing with their littermates WT). b, c No differences were detected in both total distance travelled and mean speed between groups. d Representative images of path length in open field are showed. Data are expressed as mean ± SEM, n = 4–7. Statistical significance was found by two-way ANOVA followed by Tukey’s multiple comparisons test