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. 2023 Apr 26;30:29. doi: 10.1186/s12929-023-00919-1

Table 3.

Endolysins and related derivatives active against G+ bacteria

Endolysin and/or derivative Phage Antimicrobial spectrum Enzymatic activity Type of infection treated in vivo Clinical trial phase Clinical trials identifier/accession No References
SAL200 SAP-1 S. aureus Amidase and endopeptidase Bacteremia IIa NCT03089697 [61]
Exebacase (CF-301 or PlySs2) Prophage of S. suis S. aureus, Streptococcus Peptidase Bacteremia III NCT04160468 [28, 62]
P128 CHAP domain (TAME phage K) + SH3b (lysostaphin) S. aureus CHAP Bacteremia II NCT01746654 [96–98]
Staphefekt SA.100 M23 endopeptidase (lysostaphin) + Amidase (Ply2638) + SH3b (Ply2638) S. aureus Amidase and endopeptidase Atopic dermatitis I/II NCT02840955 [64, 99]
Medolysin® – S. aureus – Bacterial wound infections – – [100]
XZ.700 Staphefekt SA.100 deleted 44 amino acids region deleted S. aureus Amidase and endopeptidase Skin infection Pre-clinical – [64]
MV-L MR11 S. aureus, S. simulans Amidase and endopeptidase Nares infection, sepsis Pre-clinical BAF33253 [81]
LysP108 P108 S. aureus Amidase Subcutaneous abscess Pre-clinical YP_009099525 [17]
80α phi80α S. aureus Amidase and endopeptidase Systemic infection Pre-clinical ABF71642 [101]
phi11 phi11 S. aureus Amidase and endopeptidase Systemic infection Pre-clinical YP_500516 [101]
LysK K S. aureus Amidase and endopeptidase Systemic infection Pre-clinical YP_024461 [101]
Ply2638 2638A S. aureus Amidase and endopeptidase Systemic infection Pre-clinical AAX90995 [101]
Twort Twort S. aureus Amidase and endopeptidase Systemic infection Pre-clinical AAX92311 [101]
phiSH2 phiSH2 prophage S. aureus Amidase and endopeptidase Systemic infection Pre-clinical BAE05642 [101]
LysWMY phiWMY S. aureus Amidase and endopeptidase Systemic infection Pre-clinical BAD83402 [101]
CHAPK K S. aureus Endopeptidase Nasal infection Pre-clinical 4CT3_D [18, 102]
ClyF CD domain (Ply187) + CBD domain (PlySs2) S. aureus CHAP Bacteremia and burn wound infection Pre-clinical - [22]
LysSS SS3e S. aureus, Salmonella, Escherichia coli – Systemic infection Pre-clinical AAW51228 [103]
Ply6A3 PD-6A3 Acinetobacter baumannii, E. coli, S. aureus – Sepsis Pre-clinical ALM01856 [104]
gp144 ΦKZ Pseudomonas aeruginosa, S. aureus, E. coli, B. cereus Transglycosylase – – AAL83045 [105]
LysGH15 GH15 Staphylococcus Amidase and CHAP Bacteremia Pre-clinical ADG26756 [26, 67, 106, 107]
PlyGRCS GRCS S. aureus, S. epidermidis Endopeptidase – – AHJ10590 [19]
ClyH CD domain (Ply187) + non-SH3b (phiNM3) S. aureus Amidase Intraperitoneal infection Pre-clinical – [108]
MR-10 MR-10 S. aureus – Subcutaneous Pre-clinical – [109, 110]
LysH5 PhiH5 S. aureus, S. epidermidis Amidase and endopeptidase – – ACE77796 [59, 68]
ClyS

CD domain (Twort) + 

CBD domain (phiNM3)

 S. aureus Endopeptidase Intraperitoneal, nasal and skin infection Pre-clinical – [73, 78]
ClyC CD domain (Ply187) + CBD domain (LysSA97) S. aureus – Bacteremia Pre-clinical – [38]
Lys16 P68 S. aureus CHAP – – AAO83890 [101, 111]
LysSAP33 SAP33 S. aureus CHAP – – QDH45454 [42]
Pal Dp-1 S. pneumoniae Amidase Nasopharyngeal infection Pre-clinical O03979 [16]
Cpl-1 Cp1 S. pneumoniae Muramidase Endocarditis, bacteremia, pneumonia, meningitis Pre-clinical NP_044837 [69, 112–114]
Cpl-7 CP-7 S. pneumoniae, S. pyogenes, E. faecalis Muramidase Embryo infection Pre-clinical P19385 [115, 116]
Cpl-711 CD domain (Cpl-7) + CBD domain (Cpl-1) S. pneumoniae Muramidase Bacteraemia Pre-clinical – [117]
PL3 CD domain (Cpl-7) + CBD domain (LytA) S. pneumoniae Amidase Embryo infection Pre-clinical – [118]
ClyJ

CD domain (PlyC) + 

CBD domain (SPSL1)

 S. pneumoniae CHAP Bacteraemia Pre-clinical – [119]
ClyJ-3 ClyJ variant S. pneumoniae CHAP Bacteraemia Pre-clinical – [120]
ClyJ-3 m ClyJ-3 variant S. pneumoniae CHAP Bacteraemia Pre-clinical – [121]
23TH_48 23TH S. pneumoniae Amidase – – QOI69927 [122]
MSlys MS1 S. pneumoniae Amidase – – AQY55407 [123]
PlyC C1 S. pyogenes Amidase Mucosal epithelium infection Pre-clinical AAP42310 [76]
PlyPy MGAS5005 prophage S. pyogenes Endopeptidase Bacteremia Pre-clinical AAM79913 [124]
PlyGBS NCTC11261 Group B streptococci, S. agalactiae Endopeptidase and muramidase Vaginal and oropharynx infection Pre-clinical AAR99416 [74, 125]
PlySK1249 SK1249 prophage S. agalactiae, Streptococcus dysgalactiae Amidase and endopeptidase Bacteremia Pre-clinical EGL49245 [126]
PlyG γ-phage B. anthracis, B. cereus Amidase Intraperitoneal infection Pre-clinical PFW40491 [24]
PlyPH

B. anthracis BA2805

genome

 B. anthracis, B. cereus – Intraperitoneal infection Pre-clinical WP_098639153 [127]
LysPBC2 PBC2 B. cereus Amidase – – AKQ08512 [128]
PlyV12 Φ1 E. faecalis, E. faecium Amidase – – YP_009814814 [70]
LysEFm5 IME-EFm5 E. faecium Amidase – – YP_009200901 [129]
LysIME-EF1 IME-EF1 E. faecalis Endopeptidase Intraperitoneal infection Pre-clinical YP_009042672 [126]
LysEF-P10 EF-P10 E. faecalis Endopeptidase Balance of the gut microbiota Pre-clinical AQT27695 [130]
Ply3626 phi3626 C. perfringens Amidase – – NP_612849 [131]
Psa St13 C. perfringens Amidase – – WP_011010276 [132]

–, unknown or data not available