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. 2023 Apr 26;24:95. doi: 10.1186/s13059-023-02945-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Functional analysis of GWAS for SA and ABA content related to apple fruit weight. Fruit weight (a), SA (b), and ABA (c) contents in high- (H) and low- (L) weight domesticated fruits. d,e Manhattan plots of SA and ABA contents by GWAS. f An unrooted phylogenetic tree was constructed for MD15G1051400 and other NAC members. Fruit weight (g), SA (h), and ABA (i) contents during fruit development. j,k qRT-PCR analysis of NAP (MD16G1069500) and ABCG25 (MD03G1222900) in H and L apple groups. l,m Results of transient overexpression of NAP and ABCG25 in apple fruits. n Nucleotide polymorphisms identified in the promoter and coding sequence of NAP. o Box plot of SA and ABA correlated with identified nucleotide polymorphism. p Nucleotide polymorphisms in H and L apple groups. DAFB, days after full bloom. Data are expressed as mean ± sd. “*” indicate p ≤ 0.05 by t-test