Extended Data Fig. 2. CD44 mediates the uptake of metals in inflammatory macrophages.
a, ICP-MS of cellular metals in aMDM treated with an anti-CD44 antibody RG7356 during activation (n = 7 donors). b, ICP-MS of cellular metals in aMDM supplemented with HA (0.6-1 MDa) or permethylated (meth-) HA during activation (n = 6 donors). c, Western blots of hyaluronan synthases (HAS) (n = 6 donors) and ATP7A/B (n = 8 donors) in MDM. d, Fluorescence microscopy of a lysosomal copper(II) probe (Lys-Cu) in aMDM under CD44 knockdown conditions (n = 5 donors). e, Fluorescence microscopy of CTR2 and Lamp2 in aMDM (n = 4 donors). f, Fluorescence microscopy of a lysosomal copper(II) probe (Lys-Cu) in aMDM under CTR2 knockdown conditions (n = 5 donors). For d–f, Scale bars, 10 μm. For a, c, d and f two-sided Mann-Whitney test. For b, Kruskal-Wallis test with Dunn’s post-test. Box plots: boxes represent interquartile range and median, and whiskers indicate the minimum and maximum values. Each colored dot represents a distinct donor for a given panel.