Fig. 7. Base flipping positions A1408 for N1-methylation by NpmA. During methylation, A1408 is flipped from its helix by NpmA and the adenosine nucleotide is stabilized by π–π stacking interactions with two tryptophan residues. The backbone carbonyl of F105 forms a hydrogen bond (red dashed line) with N6 of A1408, positioning the N1 for methylation (PDB: 4OX9). Structure image was prepared using PyMol.
