Increased GPR176 was identified in colorectal cancer. A) The cluster analysis of dysregulated mRNA in tissues obtained from CRC patients. B) Significant different expression mRNA in tumor tissues, corresponding adjacent tumor tissues, and normal tissues. C) Overlapped results combining high throughput screening data with GPCR database. D) TCGA analysis of candidate GPCRs expression in colorectal cancer tissues versus normal tissues, including 51 normal and 379 tumor samples. E) Relative expression of candidate GPCRs between normal, adjacent normal, and cancer tissues analyzed by quantitative reverse transcription‐PCR (qRT‐PCR), n = 20. F) Large cohort validated the expression of GPR176 in tissue samples obtained from CRC patients, n = 249. G) Immunohistochemistry of GPR176 in histologic section of CRC patients. The representative images were shown with scale bars of 200 µm and magnified scale bars of 50 µm. Data was presented with mean±SD, **p < 0.01 and *p < 0.05.