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. 2023 Mar 11;10(12):2205627. doi: 10.1002/advs.202205627

Figure 4.

Figure 4

GPR176 suppressed the mitophagy in CRC cells. A) Key proteins involving in cell cycle and apoptosis were assessed by western blot in cells treated with GPR176 ovexpression or knockdown lentivirus. B) Cell cycle and apoptosis‐associated protein expression in Gpr176 CKO mice. C) Pathway enrichment analysis from the DEGs highly corrected with GPR176 expression from high throughput screening. D) The conditions of mitochondria in CRC cell lines and AOM/DSS mice model assessed by electron microscopy (EM). E) Representative images of MitoTracker Red assay in GPR176 knockdown (left) and overexpression cells (right). F) Western blot of mitochondrial membrane proteins in GPR176 CKO epithelial cells from AOM/DSS mice model. G) Immunofluorescence analysis of LC3B in GPR176 knockdown (left) and overexpression cells (right). H) Western blot of mitophagy proteins in GPR176 knockdown cells. Original magnification, ×64, bar = 10 µm (E, G), ×100, bar = 0.5 µm (D).