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. 2023 Mar 11;10(12):2205627. doi: 10.1002/advs.202205627

Figure 6.

Figure 6

The recruitment of GNAS intracellularly is required for the function of GPR176.| A) cAMP level in cells treated with GPR176 and GNAS shRNA or overexpression lentivirus. B) Western blot of BNIP3L phosphorylation, Cyto, C, and LC3 II level in GPR176 overexpressed cells transfected with shGNAS or GNAS plasmid. C) cAMP level in GPR176 knockdown cells treated with constitutively active Gαs. D) proliferation analysis. E) western blot of Cyto C, LC3 II, and BNIP3L phosphorylation level. F) Co‐IP assay confirmed the binding of GPR176 and GNAS. G) Colocalization images of GPR176 and GNAS determined by immunofluorescence. H) Co‐IP assay determined the binding of exogenous His‐GPR176 and FLAG‐GNAS in 293T cells. Original magnification, ×64, bar = 10 µm G) Data was presented with mean ± SD, **p < 0.01, and *p < 0.05, ns indicated no significance.