TABLE 1.
Primers used in this study
| Primer | Sequencea | Use |
|---|---|---|
| J23119-F | GAAACAGCTATGACATGATTACGAATTCGAGCTCTGAGCTAGCTGTCAAACTGGCCGTCG | pBCG01 |
| J23119-R | AGTAATTTCATTAGGTCTTTTGAGCTCATCTTGTTGTTACCTCCTTAGCAGG | pBCG01 |
| hicA-F | GCTAAGGAGGTAACAACAAGATGAGCTCAAAAGACCTAATGAAATTACT | pBCG01 |
| hicA-R | GTATAAATGTTAGTGATGATTATAAAAATTAAAAGTATGAGGAGAACAAGCAAG | pBCG01 |
| PrpsJFP-F | GAAACAGCTATGACATGATTACGAATTCGAGCTAGCACCAGGTTTAGGGGCTG CTATAG | pBCG01 |
| PrpsJFP-R | GGCATCAAGACGATGCTGGTATCACCGGTTTAAATTAACTACATAAGATAATTCTTATG | pBCG01 |
| hicA(rpsJFP)-F | GAATTATCTTATGTAGTTAATTTAAACCGGTGATACCAGCATCGTCTTGATGCCCTTG | pBCG01 |
| hicA-R2 | AAGTATGAGGAGAACAAGCAAGGCATTAAAGCCCTGCTTGCCTG | pBCG01 |
| hicB-F | CAGGCAAGCAGGGCTTTAATGCCTTGCTTGTTCTCCTCATACTT | pBCG03 |
| hicB-R | GTATAAATGTTAGTGATGATTATAAAATTATTTTTCAATTCCTAATTCTTTT | pBCG03 |
| MSSKT-R | GTATAAATGTTAGTGATGATTATAAAATTAGGTCTTTGAGCTCATCTTGTTG | pBCG04 |
| pCWU6-F | GTCCTAGAACTAGTGAAATTTCCG | Removes repA and oriFn from pBCG02 |
| pCWU6-R | GTAGCTTCCATACATATCACACTC | Removes repA and oriFn from pBCG02 |
| TnaAupF | GAGTGTGATATGTATGGAAGCTACAGTAATATTGTTTGATGAAATTGAAAAGG | pBCG02-ΔtnaA |
| TnaAupR | TCTGCTTGTTGTTCAGGAGTTTCATCACCTAGGAACATAGCTGACCATTG | pBCG02-ΔtnaA |
| TnaAdnF | GCTATGTTCCTAGGTGATGAAACTCCTGAACAACAAGCAGAAGTTGCAAATG | pBCG02-ΔtnaA |
| TnaAdnR | CGGAAATTTCACTAGTTCTAGGACAGCAGCAAGCAATGCTCCAAGTGGACAAATATATATAG | pBCG02-ΔtnaA |
| RadDupF | GAGTGTGATATGTATGGAAGCTACATAAATTTAATTTAACTTATTAATTATAAGG | pBCG02-ΔradD |
| RadDupR | GCATCCTTACCTGACATTTTTACTGCTTCATCTGCATAAGAAAAACCACCATTAAT | pBCG02-ΔradD |
| RadDdnF | GATGAAGCAGTAAAAATGTCAGGTAAGGATGCTATTGGTATTT | pBCG02-ΔradD |
| RadDdnR | CGGAAATTTCACTAGTTCTAGGACTCCTGATTCATTTATTGCTTCACCATCTACT | pBCG02-ΔradD |
| ΔradD-F | GAGTTTCAGATGAAATGAATGCCCTACAAG | Confirms radD deletion |
| ΔradD-R | CTGCAGTAGTTGCTCCACCTTTTACAGCTG | Confirms radD deletion |
a
Underlined are primer sequences specific to the ends of the corresponding assembly.