FIG 3.

Accumulation and efflux of CF in LcS. Accumulation and efflux of CF were evaluated by time-lapse analysis of CF fluorescence by FCM. (A) LcS cells in MRS pure culture were washed with DPBS to remove saccharide, and then cFDA was added. The LcS cells were immediately analyzed by FCM, and the fluorescence of CF was monitored for 20 min. (B) LcS cells with CF accumulation were prepared by the same cFDA treatment for 15 min, and then 10 mM glucose was added. The fluorescence of CF was monitored by FCM for 15 min. (C) CF efflux was similarly evaluated with the addition of glucose at 0.001 mM to 1 mM.