FIG 1.

tdFAST2-expressing methanogens show a specific fluorescence in the presence of HBR-3,5DOM. Fluorescence of M. acetivorans and M. maripaludis cells is shown. (a) Fluorescence spectrum upon excitation at 515 nm. (b) Fluorescence at 590 nm when different excitation wavelengths are applied. Cells expressing tdFAST2 (tdFAST2) or control cells carrying an empty vector construct (e.v.) were analyzed in presence (w/ fluorogen) or absence (w/o fluorogen) of HBR-3,5DOM. Cells were in the stationary growth phase. Mean values and standard deviation of triplicates are shown.