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M. acetivorans fluorescence changes at different growth phases. M. acetivorans cells expressing tdFAST2 (+) or harboring an empty vector construct (–) were analyzed at exponential (Exp; OD[600] 0.3–0.6), late exponential (Late Exp; 0.8 to 1.1), and stationary growth (Stat; >1.1). tdFAST2:HBR-3,5DOM fluorescence (λEx = 515 nm/λEm = 590 nm), and autofluorescence (λEx = 420 nm/λEm = 480 nm) was measured. Technical duplicates are shown.
