Figure 3. The effect of calcineurin inhibitors on Kir4.1/Kir5.1 is absent in the DCT treated with PP1 or H₂O₂.

A single-channel recording demonstrates the effect of FK506 and CsA on the 40 pS K⁺ channels in the DCT pretreated with 100 nM PP1, an inhibitor of SFK (A) or pretreated with 100 μm H₂O₂ (B). Four parts of the trace (indicated by numbers) are extended to show the fast resolution. The experiments were performed in cell-attached patches (male mice). The results of the experiments are summarized in 2 bar graphs with scatterplots at the bottom of each panel. The DCT was bathed in a solution containing 140 mM NaCl/5 mM KCl, and the pipette solution contained 145 mM K⁺. Significance is determined by 1-way ANOVA.