Figure 2.

Diversity across atlas of bacterial AzoRs and activity of purified proteins. (a) Sequence Similarity Network of AzoR proteins across IGC and IBDMDB including reference sequences used to identify putative AzoRs colored according to Phyla, with abbreviations shown for proteins used in this study (E=1×10⁻⁴⁰). (b) Catalytic efficiencies for Methyl Red azo reduction by purified AzoR enzymes as a function of hydride donor present in reaction. (c) Catalytic efficiencies for Amaranth azo reduction by purified AzoR enzymes. Statistical comparisons reflect activity of CmAzoR compared to other enzymes and were calculated using Tukey’s multiple comparisons test. *P < 0.05, ****P < 0.0001.