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. 2023 Apr 26;14:106. doi: 10.1186/s13287-023-03305-8

Fig. 2.

Fig. 2

Characterization of human-induced pluripotent stem cells (hiPSCs) derived from three different sources. A Quantitative real-time polymerase chain reaction (qRT-PCR) results of the reprogramming factors OCT4, SOX2, c-MYC, KLF4, and NANOG. B Immunofluorescence assay results of the pluripotency markers, TRA1-60, SSEA4, SOX4, SOX2, and NANOG. C Flow cytometric analysis results of TRA-1-60 and SSEA4. D qRT-PCR results of the three germ layer markers, Brachyury (mesoderm), SOX17 (endoderm), and Nestin (ectoderm). E Karyotyping results. Abbreviations: PB, peripheral blood; CB, cord blood; BM, bone marrow