KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti Arhgap11a | Bethyl | Cat#A303–097A, RRID# AB_10890540 |
| Rabbit anti Arhgap11a | Abcam | Cat#ab113261, RRID# AB_10866587 |
| Mouse anti Nestin | BD biosciences | Cat#556309, RRID# AB_396354 |
| Rabbit anti Tbr1 | Abcam | Cat#ab31940, RRID# AB_2200219 |
| Rat anti Ctip2 | Abcam | Cat#ab18465, RRID# AB_2064130 |
| Mouse anti ROR-beta | R&D Systems | Cat#N7927, RRID# AB_1964364 |
| Rabbit anti Laminin | Millipore | Cat#AB2034, RRID# AB_91209 |
| Rabbit anti Calretinin | Swant | Cat#7697, RRID# AB_2721226 |
| Rat anti Sox2 | Thermo Fisher | Cat#14–9811-80, RRID# AB_11219070 |
| Rabbit anti Tbr2 | Abcam | Cat#ab183991, RRID# AB_2721040 |
| Rabbit anti Ki67 | Cell Signaling Technology | Cat#12202, RRID# AB_2620142 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | JAX | Cat#000664, RRID# IMSR_JAX:000664 |
| Mouse: Dlx-Cre (Tg(dlx5a-cre)1Mekk/J) | JAX | Cat#008199, RRID# IMSR_JAX:008199 |
| Mouse: Arhgap11aem1(IMPC)J | KOMP | Cat#MGI:5907256, RRID# MMRRC_042323-JAX |
| Oligonucleotides | ||
| siRNA: siRNA flex targetting Arhgap11a | Qiagen | Cat#SI00902111 |
| siRNA: siRNA flex targetting Arhgap11a | Qiagen | Cat#SI00902132 |
| siRNA: siRNA flex targetting Arhgap11a | Qiagen | Cat#SI00902118 |
| siRNA: All Stars Negative Control | Qiagen | Cat#1027281 |
| In situ hybridization probe target human ARHGAP11A |
This paper | 2117–2657 of human ARHGAP11A (NM_014783.5) |
| smFISH Probe Set: Arhgap11a | LGC Biosearch Technologies | Custom made |
| smFISH Probe Set: MS2 | LGC Biosearch Technologies | Pilaz et al., 2016 |
| smFISH Probe Set: Dendra | LGC Biosearch Technologies | Pilaz et al., 2016 |
| qPCR primer: Actb FW | Thermo Fisher | AGATCAAGATCATTGCTCCT |
| qPCR primer: Actb Rev | Thermo Fisher | CCTGCTTGCTGATCCACATC |
| qPCR primer: Pax6 FW | Thermo Fisher | TCTTTGCTTGGGAAATCCG |
| qPCR primer: Pax6 Rev | Thermo Fisher | CTGCCCGTTCAACATCCTTAG |
| qPCR primer: Arhgap11a FW | Thermo Fisher | GCAGGTGTGCCAAGGCGAAGT |
| qPCR primer: Arhgap11a Rev | Thermo Fisher | TGCAAGTCGCCAACCAACACTTTCA |
| Taqman probe: Tubb3 | Thermo Fisher | Cat#Mm00727586_s1, RRID# |
| Taqman probe: Gapdh | Thermo Fisher | Gapdh |
| Recombinant DNA | ||
| Plasmid: pCAGGS-GFP | Gift from Dr. Nicholas Gaiano |
Mizutani et al., 2007 |
| Plasmid: pGLAST-EGFP-CAXX | Gift from Dr. Tarik Haydar |
Gal et al., 2006 |
| Plasmid: pCAGGS-PB-mCherryCAXX | Gift from Dr. Cagla Eroglu | N/A |
| Plasmid: pCAGGS-EX | Gift from Dr. Nicholas Gaiano |
Mizutani et al., 2007 |
| Plasmid: pCAGGS-EGFP-nls | This paper | N/A |
| Plasmid: pCAGGS-EGFP-nls-Arhgap11a 3’UTR | This paper | N/A |
| Plasmid: pCAGGS-EGFP-nls-Arhgap11a 5’UTR | This paper | N/A |
| Plasmid: pCAGGS-EGFP-nls-Arhgap11a CDS | This paper | N/A |
| Plasmid: pCAGGS-Dendra2 | Dr. Debra Silver Lab | Pilaz et al., 2016 |
| Plasmid: pCAGGS-Dendra2-Arhgap11a-noUTR | This paper | N/A |
| Plasmid: pCAGGS-Dendra2-Arhgap11a-5’UTR | This paper | N/A |
| Plasmid: pCAGGS-5’UTRArhgap11a-Dendra2 | This paper | N/A |
| Plasmid: pCAGGS-Arhgap11a(GD)-Dendra-5’UTR | This paper | N/A |
| Plasmid: pCAGGS-CFP-MS2-noUTR | Dr. Debra Silver Lab | Pilaz et al., 2016 |
| Plasmid: pCAGGS-CFP-MS2-Arhgap11a 5’UTR | Dr. Debra Silver Lab | Pilaz et al., 2016 |
| Software and Algorithms | ||
| FIJI | NIH | https://ImageJ.NIH.gov/IJ |
| GraphPad Prism (9) | GraphPad | https://www.graphpad.com/scientificsoftware/prism/ |