Figure 3.

Molar proportion of acetate of rumen-cannulated Bos indicus Nellore steers receiving forage-based diets supplemented or not (CON, n = 8) with 13 mg/kg of dry matter (DM) of narasin (NAR, n = 8; Zimprova; Elanco Animal Health, São Paulo, SP, Brazil), 20 mg/kg of DM of lasalocid (LAS; n = 8; Taurotec; Zoetis, Sao Paulo, SP, Brazil), or 20 mg/kg of DM of virginiamycin (VRM; n = 8; V-Max; Phibro Animal Health Corporation, Guarulhos, SP, Brazil) for 140 days (Exp. 1). Treatments were offered daily throughout the experimental period (day 0 to 140). Rumen samples were collected on day 0 (prior to first treatment administration), 28, 56, 84, 112, and 140 of the study. Data were analyzed using results from day 0 as an independent covariate. Within days, letters indicate treatment comparisons (P ≤ 0.05): a = CON vs. NAR, b = CON vs. LAS, c = CON vs. VRM, d = NAR vs. LAS, e = NAR vs. VRM, and f = LAS vs. VRM.