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. 2023 Apr 12;616(7958):814–821. doi: 10.1038/s41586-023-05922-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — (a–c) Schematic representation of the 4SU-DRB labelling (a), TUC conversion (b) and elongation rate calculation (c). Cell culture and eppendorf icons created with BioRender.com. (d) Percentage of mismatches in every time point of the experiment (0 mins, 15 mins, 30 mins, 45 mins) in one of the proliferating replicates. There is a noticeable increase in A-to-G and T-to-C mismatches in the last two time points.