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. 2023 Apr 3;5(4):699–709. doi: 10.1038/s42255-023-00769-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Time course of relative abundance of TG;Y1 species upon labelling with FA 18:2;Y. Relative amounts of all TG;Y1 species of the pulse-chase experiment are shown colour-encoded. Each row represents one species. b, MS2 analysis of one species (open arrow) at chase time 48 h. The precursor peak at m/z 1,025.8 is omitted. The four series of fragment peaks indicate the FA composition 16:1_18:1_18:2;Y. c, MS2 analysis of one species (closed arrow) at chase time 48 h. The precursor peak at m/z 1,021.8 is omitted. The three series of fragment peaks indicate the FA composition 16:1_16:1_20:4;Y. Note that in b and c the actual incorporated FA;Ys are directly identified from the peak masses and printed in red. In a, numbers are encoded by intensity of colour (min–max of the entire data block), derived from averages, n = 12. Detailed statistical data of this experiment are shown in the Supplementary Table 2.