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. 2023 Apr 12;616(7958):764–773. doi: 10.1038/s41586-023-05927-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a. Violin plot from DropViz.org showing the expression of Crym in astrocytes, neurons, endothelial cells, and myelin associated cells of the striatum. Center of the box plot for all violin plots represent the median, the box limits indicate the first and third quartiles, and the whiskers/range indicate the distribution of the data range. b. Bar graphs denoting the total number of Crym mRNA puncta per tdTomato+ astrocyte and S100β+ cell. Mean and SEM are shown (n = 7 cells from 4 mice per group). c. Representative image of an Aldh1l1 tdTomato+ striatal astrocyte with Crym mRNA puncta expression (green). Arrow denotes puncta within the cell. d. Representative image of S100β+ cells with Crym mRNA puncta expression (green). Arrows denote the puncta within the cells. e. Violin plot from DropViz.org showing the expression of Tjp1 in astrocytes, neurons, endothelial cells, and myelin associated cells of the striatum. f. Bar graphs denoting the total number of Tjp1 mRNA puncta per tdTomato+ astrocyte and S100β+ cell. Mean and SEM are shown (n = 7 cells from 4 mice per group) g. Representative image of an Aldh1l1 tdTomato+ striatal astrocyte with Tjp1 mRNA puncta expression (green). Arrows denote puncta within the cell. h. Representative image of S100β+ cells with Tjp1 mRNA puncta expression (green). Arrows denote the puncta within the cells. i. Violin plot from DropViz.org showing the expression of Mapt in astrocytes, neurons, endothelial cells, and myelin associated cells of the striatum. j. Bar graphs denoting the total number of Mapt mRNA puncta per tdTomato+ astrocyte and S100β+ cell. Mean and SEM are shown (n = 7 cells from 4 mice per group). k. Representative image of an Aldh1l1 tdTomato+ striatal astrocyte with Mapt mRNA puncta expression (green). Arrow denotes puncta within the cell. l. Representative image of an S100β+ cell with Mapt mRNA puncta expression (green). Arrow denotes the puncta within the cell.